IdealPhotonics' miniature two-photon microscope is a compact microscopic imaging system based on the principle of nonlinear optical excitation. It achieves high-resolution (submicron) fluorescence imaging of deep tissues (penetration depth >1mm) using femtosecond lasers (wavelength range 800-1300nm). Its innovative modular optical design and lightweight structure (weight <5kg) support real-time observation of the brain nerves of freely moving animals (such as mice), making it a portable high-end imaging tool in neuroscience, in vivo tumor research, and drug development.Including femtosecond laser wavelength (tunable from 800-1300nm), imaging resolution (0.5μm laterally/2μm axially), imaging depth (>1.2mm), and system weight (<3kg), its innovative "fiber-coupled laser module" and "miniature scanning galvanometer" boost imaging speed to 30fps (512×512 pixels). It redefines the lightweight limits of in vivo microscopy in observation of cortical neurons in freely moving mice (calcium signal capture delay <10ms), dynamic monitoring of tumor microcirculation (blood flow velocity accuracy ±0.1mm/s), and portable field biological research (endurance >8h).